Table 1.

Summary of clinical history, H&E staining, and FBN3 staining in control and PCOS ovaries

Case number	Age	Clinical history of PCOS	History comments	H&E staining	FBN3 staining
1	43	No	Endometrial endometrioid CA, FIGO 1a	TF, AnF, GF, FC, FL, CAL	++
2	19	No	Salpingitis	TF to large AnF, AtF, foci of CF	+/−
3	17	No	Megacolon, rectovaginal fistula	Scant cortical tissue with cautery and surface fibrous adhesions. TF, AnF, GF clustered in focal area	+/−
4	29	No	Opposite ovary with mucinous cystadenoma	CL, AnF, TF, large FC	++
5	30	No	Placenta percreta, C/S hysterectomy	Many TF, AnF, FL, CAL	++
6	34	No	Endometriosis	Many TF, AnF, corpus hemorrhagicum	No
7	34	No	PSO; BRCA mutation detected	Wide range of follicle development	++
8	31	No	Corpus luteum—persistent ovarian cyst	Active folliculogenesis, TF to GF	++
9	27	No	Endometriosis	TF and multiple AnF	++
10	40	No	Irregular menses, DM, HTN, hypothyroid, endometrial endometrioid CA, FIGO I	TF, multiple FC and AnF, FL	++
11	38	No	Irregular menses, atypical hyperplasia	TF, AnF, GF, CL, FC, AtF, FL	++
12	38	No	IDM since 8 years old, hypothyroid, HTN, HCh, uterine CA	TF, AnF, FC, FL, AtF	+
13	27	No	IDM	TF, AnF, GF, FL	++
14	40	No	Endometrial CA, DM, HCh	TF, AnF, FC, FL, AtF, CF	+
15	34	No	Breast carcinoma	TF, AtF, CAL	++
16	45	No	Cervical dysplasia	TF, AtF, CAL	++
17	34	No	PSO; BRCA mutation detected	Range of follicles present—TF to GF, and FC	++
18	40	No	PSO; BRCA mutation detected	TF, FC, CF	++
19	45	No	Breast carcinoma	Senescent, AtF, CAL, rare TF	++a
20	46	No	PSO; BRCA mutation detected	Senescent, CAL, rare CEI, endometriosis	++a
21	51	No	Breast carcinoma. PSO; BRCA mutation detected	Senescent, focal CEI, AtF, CAL	++a
22	46	No	Strong family history breast and ovarian CA, no BRCA mutation detected	Senescent, AtF, FC	++a
23	38	Yes	Pulmonic stenosis, DM, enlarged ovary with cysts on ultrasound	Stromal hyperplasia, minimal HT, full follicle range, rare FC, SF	+
24	35	Yes	Oligomenorrhea, infertility, insulin resistance, obesity	Range of follicles present—TF to GF, AtF, and few FC	+/−
25	17	Yes	Amenorrhea, HTN, hirsuitism, obesity	Multiple FC, rare AnF, scattered TF, HT, SF	No
26	24	Yes	No additional history	Multiple FC, TF, rare GF	No
27	34	Yes	No additional history	TF, numerous large AnF, FL, SF	No
28	37	Yes	Infertile, hirsute, HTN	Range of follicles present—TF to GF, FC, SF, CF, FL. No HT	+
29	39	Yes	No additional history	FC, GF, TF, FL, SF. No HT	No
30	19	Yes	Oligomenorrhea, hirsuitism, obesity	TF, multiple AtF, FC, SF, CF	No
31	45	Yes	Irregular menses	Histologically normal ovary with active folliculogenesis	++

^aFBN3 staining focal in cortical stroma without identifiable follicle.

H&E, hematoxylin and eosin; PCOS, polycystic ovary syndrome; CA, carcinoma; TF, transitional follicle (primordial to primary); AnF, antral follicle; GF, Graafian follicle; FC, follicle cyst; FL, folliculolysis; CAL, corpus albicans; AtF, atretic follicle; CF, cortical fibrosis; CL, corpus luteum; C/S, cesarean section; PSO, prophylactic salpingo-oophorectomy; DM, diabetes mellitus; HTN, arterial hypertension; IDM, insulin-dependent diabetes mellitus; HCh, hypercholesterolemia; CEI, cortical epithelial inclusion; HT, hyperthecosis; SF, subcapsular fibrosis.