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. Author manuscript; available in PMC: 2010 Sep 20.
Published in final edited form as: Mech Ageing Dev. 2009 Nov–Dec;130(11-12):777–783. doi: 10.1016/j.mad.2009.10.003

Figure 7. The association of Hsp90 with proteasome complexes is altered by aging and dietary restriction.

Figure 7

The levels of Hsp 90 in the crude lysates (A) of liver and brain were analyzed by immunoblotting with antibodies against Anti-Hsp90. Levels of Hsp 90 associated with 20S proteasomes in liver and brain lysates (B) were analyzed by immunoprecipitation with 20S proteasome antibodies against core subunits and immunoblotting with antibodies against Hsp90. Ctrl: control experiment where the lysates were incubated with beads alone without antibody for the background. 3 M: 3 month AL; 25 M: 25 month AL; DR, 25 month dietary restricted.