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. Author manuscript; available in PMC: 2010 Sep 20.
Published in final edited form as: Am J Physiol Lung Cell Mol Physiol. 2002 Mar;282(3):L405–L410. doi: 10.1152/ajplung.2002.282.3.L405

Figure 2. Semi-quantitative RT-PCR of RNA extracts from fetal rat lung fibroblasts and WI38 human embryonic lung fibroblasts.

Figure 2

Primary fetal rat lung fibroblasts (E18) and WI38 human embryonic lung fibroblasts were treated with either dexamethasone (DEX, 1 × 10−8 M), parathyroid hormone-related protein (PTHrP, 5 × 10−7 M), or prostaglandin E2 (PGE2, 5 × 10−7 M) for 24 hours and subsequently analyzed for leptin mRNA expression using semi-quantitative RT-PCR. Co-amplification with GAPDH cDNA was used as an internal standard for normalization of the aliquot volumes for leptin mRNA expression. (Center panel) Representative blots for RT-PCR of leptin mRNA; the top and bottom graphs are densitometric quantitation of the corresponding blots. Each bar represents the mean ±standard deviation, n=5, ***p<.0001 by analysis of variance.