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. Author manuscript; available in PMC: 2010 Sep 20.
Published in final edited form as: Am J Physiol Lung Cell Mol Physiol. 2002 Mar;282(3):L405–L410. doi: 10.1152/ajplung.2002.282.3.L405

Figure 4. Fetal rat lung fibroblasts secrete leptin under the influence of PTHrP.

Figure 4

E21 fetal rat lung fibroblasts were cultured in 2 cm2 wells in serum-free DMEM containing 0.1% fatty acid-free bovine serum albumin for up to 6 hours plus or minus parathyroid hormone-related protein (PTHrP, 5 × 10−7 M). Leptin concentrations were determined by an enzyme linked immunosorbent assay for rat leptin. Each bar represents the mean±standard deviation, n=5, *p<.05, **p<.01, ***p<.001 by analysis of variance.