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. Author manuscript; available in PMC: 2010 Sep 20.
Published in final edited form as: Am J Physiol Lung Cell Mol Physiol. 2002 Mar;282(3):L405–L410. doi: 10.1152/ajplung.2002.282.3.L405

Figure 8. Leptin mRNA expression is PTHrP-dependent.

Figure 8

E18 fetal rat lung explants were maintained in culture for 3 days plus or minus PTHrP receptor antagonist (PTHrP Ant., 1 × 10−6 M). The explants were subsequently analyzed for leptin mRNA expression by semi-quantitative RT-PCR. Co-amplification with GAPDH cDNA was used as an internal standard for normalization of the aliquot volumes for leptin mRNA expression. The graph represents densitometric quantitation of the leptin mRNA bands; each bar is the mean±standard deviation, n=5,***p<.0001 by analysis of variance.