Figure 2.—

CGH microarray analysis of DNA derived from four spores of a tetrad derived from the triploid MH10. (A) Analysis of the MH10-32 tetrad by CGH microarray. DNA was isolated from individual spore cultures and labeled with Cy5-tagged nucleotides. DNA from the isogenic haploid strain was labeled with Cy3-tagged nucleotides. The individual samples from spore cultures were mixed with the labeled control DNA and the mixture was hybridized to microarrays containing all ORFs and intergenic region. Each chromosome is depicted by a horizontal line whose length is proportional to the length of the chromosome. Red indicates gene amplification relative to the reference strain. The meiotic sister pairs of spores are MH10-32a/MH10-32c and MH10-32b/MH10-32d. Extensive loss of disomic chromosomes is evident in spore MH10-32b. (B) Photograph of MH10-32 spore colonies on the tetrad dissection plate.