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. 2010 Sep 20;5(9):e12830. doi: 10.1371/journal.pone.0012830

Figure 4. TSD sample extracts can be used to confirm the presence of human blood and obtain the DNA profile of the bloodstain donor.

Figure 4

The use of a portable spectrophotometer (NanoPhotometer™) permits the rapid identification of the presence of blood via its characteristic Hb absorption spectrum (A, left panel). Further confirmation of the presence of blood and its human origin was provided by a positive result with an immunochromatographic test (ABAcard® HemaTrace®) (A, right panel) using an aliquot of the same TSD extract. DNA was isolated from the remaining extract and a full autosomal DNA profile (PowerPlex® 16 HS, Promega) was obtained using standard amplification conditions (B).