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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Nat Chem Biol. 2010 Aug 22;6(10):774–781. doi: 10.1038/nchembio.424

Figure 2. rNMPs in genomic DNA of Pol ε strains ± RNase H2.

Figure 2

(a) Alkali sensitivity of yeast genomic DNA. The marker lanes (M) are DNA fragments whose lengths in kilobase pairs are 12, 11, 10, 9, 8.1, 7.1, 6.1, 5.1, 4.1, 3.1, 2.0, 1.6, 1.0 and 0.52. (b) Cleavage of a double-stranded substrate containing a single ribonucleotide by yeast extracts. The position of the single ribonucleotide (rA) in the double-stranded oligonucleotide substrate is underlined. The 5′-end-labeled strand is indicated by and asterisk, S is substrate and P is cleavage product.