FIG. 6.
Inhibition of survival signaling and clonogenicity in SUM19-CER cell line by COX-2 knockdown. A. SUM149-CER cell line at passage 6 was transfected with a negative control siRNA or COX-2 specific siRNA and subjected to western blot analysis as described in Materials and Methods. B. We performed a clonogenicity assay with SUM149-CER cells transfected with a negative control siRNA or COX-2 specific siRNA in parallel with the western blotting experiment shown in panel A. Representative western blotting and clonogenicity assay results obtained in two experiments are shown.