Figure 5. MiR-99b, miR-125a-5p and miR-1269 determine intestinal epithelial cell fate.

A, B) Determination of the ideal frequency to measure resistance of HT29-Cl.19A (A) and Caco2-BBE cells (B) by scanning of the resistance using different frequencies on ECIS 8W1E electrodes with confluent cells or without cells (naked electrodes). The ratio of the log of resistance with cells over the log of resistance without cells was calculated, and the frequency at which this ratio reached a maximum value was determined as the ideal frequency. C, D) Cells were seeded on ECIS electrodes at a density of 2 × 10⁴ cells/400 μl/electrode. Resistance of HT29-Cl.19A cells was measured at 2000 Hz (C), and resistance of Caco2-BBE cells was measured at 500 Hz (D). E) Caco2-BBE cells were transfected with vehicle (grey line) or a mixture of mature miR-99b, mature miR-125a-5p and anti-miR-1269 (black line), and cell resistance was measured in real-time. F) Caco2-BBE cells were trypsinised, transfected with vehicle or with a mixture of miR-99b, miR-125a-5p and anti-miR-1269, and seeded on filters. TER of the cells was measured daily with an equilibrated Epithelial Voltohmmeter. Values were subtracted from TER value of blank filters (without cells) and were expressed as Ω.cm². Data represent means ± S.E.M. of n = 6/condition. *P < 0.05; **P < 0.005; ***P < 0.001.