Fig. 3.
Kinetic analysis of the inhibition of rat pol β by PGG. (A and B) Lineweaver–Burk double-reciprocal plots obtained by varying DNA template-primer (i.e., poly(dA)/oligo(dT)18) concentrations (A), and dNTP substrate (i.e., dTTP) concentrations (B). (C and D) The inhibition constant (Ki) were determined as 20.1 and 23.8 nM from a Dixon plot made on the basis of the same data for A and B, respectively. The amount of pol β in the assay mixture was 0.05 unit.