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. Author manuscript; available in PMC: 2011 Sep 10.
Published in final edited form as: Biochem Biophys Res Commun. 2010 Aug 5;400(1):16–20. doi: 10.1016/j.bbrc.2010.07.126

Fig. 1.

Fig. 1

Conventional PKC positively regulates TCR-induced phosphorylation of Akt. (A) T cells purified from C57BL/6 (B6) or PKC-θ−/− mice were stimulated with anti-CD3 (1μg/ml) for the indicated time points and lysed in RIPA buffer. Proteins were subjected to immunoblotting using a specific phospho-antibody against Akt Ser473. The membrane was stripped and reprobed with anti-Akt. (B) T cells from B6 mice were pretreated with pan-PKC inhibitor BIM (5 μM) for 1 h at 37 °C, then stimulated with anti-CD3 and lysed. Phospho-Akt (Ser473) and total Akt were detected as in (A). (C) T cells purified from B6 mice were pretreated with the selective conventional PKC inhibitor Gö6976 (1 μM) or an equal volume of DMSO at 37°C for 1 h, then stimulated and analyzed as in (A) and (B). Data shown represent one of three independent experiments.