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. Author manuscript; available in PMC: 2011 Sep 10.
Published in final edited form as: Biochem Biophys Res Commun. 2010 Aug 5;400(1):16–20. doi: 10.1016/j.bbrc.2010.07.126

Fig. 2.

Fig. 2

PKC-α is the PDK-2 responsible for phosphorylating Akt at Ser473 upon TCR stimulation. T cells isolated from B6 mice were stimulated with anti-CD3 and lysed. PKC-α (A) or PKC-β (B) were immunoprecipitated and in vitro kinase assays were carried out as described in Methods. The phosphorylation of Akt at Ser473 was detected by immunoblotting as described above. The membranes were stripped and reprobed with anti-PKC-α (A) or PKC-β (B). Data shown represent one of four independent experiments. **P<0.01 with respect to the IgG basal level.