FIGURE 2.

RNA-Seq coverage for the major xylanolytic gene cluster in P. bryantii B₁4. P. bryantii B₁4 was grown with either WAX or a XA as the sole carbohydrate source, and the transcriptomes were analyzed by RNA-Seq as described under “Experimental Procedures.” A detailed view of the nucleotide coverage for the major xylanolytic gene cluster in P. bryantii B₁4 during growth on WAX (red) or XA (green) is shown for two biological replicates (WAX1, WAX2; XA1, XA2). The portion of the gene cluster including ORF1907–1912 has been studied previously by Flint and co-workers (7). Asterisks denote genes for which biochemical activities have been demonstrated for their cognate gene products: xynB and xynA (7) and xynR (5).