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. 2010 Jul 23;285(39):30069–30078. doi: 10.1074/jbc.M110.148288

FIGURE 2.

FIGURE 2.

Co-expression of KLB and FGFR4 also restricts nonhepatic cell population growth. A, cell morphology. Untransfected T-Rex-293 cells (293), cells stably transfected with KLB (cKLB), cells transfected with inducible FGFR4 cDNA (iFGFR4), and cKLB cells bearing inducible FGFR4 cDNA were examined by light microscopy after exposure overnight to 1 μg/ml Tet and 300 ng/ml of FGF19. B, population growth rates. The data are cell numbers after 5 days of culture. FGF19 or FGF1 was present at 300 ng/ml where indicated. The data are the means ± S.D. from three independent experiments. (R4), cells bearing inducible FGFR4 cDNA in Tet-free medium. C and D, co-expression of KLB and FGFR4 inhibits malignant prostate tumor cell growth. The induction of FGFR4 and effect on population dynamics of AT3 cells was determined as described for 293 cells. Expression of KLB and FGFR4 were measured by immunoblotting of whole cell lysates. β-Actin was used as the loading control.