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KLB-dependent apoptosis is proportional to FGFR4 expression. A, apoptotic cell death after induction of FGFR4 for 24 h in 293 cells. FGFR4 was induced in KLB-expressing cells by the indicated concentrations of Tet. Apoptosis was monitored by flow cytometric analysis of membrane phospholipid exposure and propidium iodide uptake. Expression of FGFR4 and KLB were assessed by immunoblotting. B, rates of apoptosis in KLB-expressing 293 cells after induction of FGFR4 for 3 days.
