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YKL-40 protein is secreted from bronchial epithelial cells. A, YKL-40 protein secreted from NHBE cells was detected by Western blot analysis. Recombinant human YKL-40 was used as a positive control. B, Coomassie Blue R250 staining of PVDF membrane after chemiluminescence detection is shown. C, shown is Coomassie Blue R250 staining of original SDS-PAGE stained after transferring proteins to the PVDF membrane.
