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Scanning electron microscopy of an MSMEG_6394 conditional knock-out. Bacteria were cultured on LB agar for 5 days prior to processing for scanning electron microscopy (see “Materials and Methods”). Wild-type M. smegmatis mc2155 strain containing the kanamycin resistance plasmid pMV261 was grown at 30 °C (A) or 42 °C (B). Conditional knock-out strain 6394CKO was grown at 30 °C (C) or 42 °C (D). All panels are the same magnification, and a scale bar is in the bottom right corner.
