FIGURE 5.

Peptide recognition by Chlamydia ArtJ antisera. Two peptide arrays (8 peptides per row) were designed by synthesizing overlapping sequences of 15-residue peptides corresponding to CPn (A and D) and CT (B and C) ArtJ on a cellulose membrane and tested for binding by immunoblotting with mouse anti-CPn (A and B) and anti-CT (C and D) ArtJ polyclonal antibodies. Membranes incubated with an unrelated antiserum were used as negative controls. Numbers on the right of each panel mark the first peptide of each row. Red box in panel A indicates the CPn ArtJ region containing highly immunogenic peptides.