FIGURE 1.

CTS targets to peroxisomes in yeast. A light mitochondrial fraction was prepared from oleate-grown BSL1-11B cells transformed with a CTS expression plasmid (CTS/pRS416-GPD) or with non-recombinant vector (control) and separated on an Iodixanol density gradient. Mitochondrial and peroxisomal fractions from the gradient along with total and cytosolic samples were separated by SDS-PAGE (30 μg of protein/lane), stained with Coomassie Blue (upper panel) or immunoblotted with antibodies specific for CTS, thiolase (peroxisomal marker), and mitochondrial AAC (mitochondrial marker). The mobilities of marker proteins of known mass (kDa) are indicated on the left-hand side of the figure.