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. 2010 Jul 22;285(39):30340–30346. doi: 10.1074/jbc.M110.128868

FIGURE 5.

FIGURE 5.

Sar1D198A selectively excludes GalT2 from COPII vesicles. Cells co-expressing Sar1 (A) or Sar1D198A (B) (blue) and GalT2 (A and B) or VSV-G (C) (green) were cultured in the presence or absence of the serine/threonine kinase inhibitor H89, as indicated at left, and immunostained for Sec23 (red). The fourth column is the blue-green-red merging, with insets corresponding to higher magnifications of the boxed areas. A, cells co-expressing GalT2 and Sar1-CFP. The arrowhead in each inset points to GalT2 not co-localizing with Sec23 in the −H89 condition and co-localizing with Sec23 (and with Sar1-CFP) in the +H89 condition. B, cells co-expressing Sar1D198A-CFP and GalT2. The arrowhead in each inset marks the lack of co-localization of GalT2 (arrowhead) and Sec23 (arrow) both in the −H89 and the +H89 condition. Note the co-localization of Sar1D198A-CFP and Sec23 in the +H89 condition. C, cells co-expressing Sar1D198A-CFP and VSV-G-YFP. The arrowhead points to VSV-G co-localizing with Sec23 and Sar1D198A-CFP in the +H89 condition.