FIGURE 5.
Atg34p mutants defective in interaction with Atg8p are not enclosed in autophagosomes. Cells chromosomally expressing each GFP fusion protein were cultured in SD + casamino acid medium to a density of approximately A600 = 1.5 and treated with rapamycin for 6 h before fluorescence microscopy. Atg34p-GFP (GYS390), Atg34ΔC4p-GFP (GYS805), Atg34AEEAp-GFP (GYS822), Atg34p-GFP atg11Δatg17Δ (GYS812), and Atg34ΔC4p-GFP atg11Δatg17Δ (GYS820) strains were used. For comparison of the fluorescence intensity, Atg34p-GFP images are shown using the same brightness and contrast. The scale bar represents 5 μm.