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. 2010 Jul 16;285(39):30019–30025. doi: 10.1074/jbc.M110.143511

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Atg34p mutants defective in interaction with Atg8p are not enclosed in autophagosomes. Cells chromosomally expressing each GFP fusion protein were cultured in SD + casamino acid medium to a density of approximately A₆₀₀ = 1.5 and treated with rapamycin for 6 h before fluorescence microscopy. Atg34p-GFP (GYS390), Atg34ΔC4p-GFP (GYS805), Atg34^AEEAp-GFP (GYS822), Atg34p-GFP atg11Δatg17Δ (GYS812), and Atg34ΔC4p-GFP atg11Δatg17Δ (GYS820) strains were used. For comparison of the fluorescence intensity, Atg34p-GFP images are shown using the same brightness and contrast. The scale bar represents 5 μm.