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Kinetic analysis of OsGH3–8 with auxin analogs
Reactions were performed as described under “Experimental Procedures.” All kinetic parameters are expressed as the mean ± S.E. for n = 3.
| Substrate | V/Et | Km | kcat/Km |
|---|---|---|---|
| min−1 | μm | m−1s−1 | |
| Indole-3-butyric acid | 65.2 ± 4.7 | 2,700 ± 410 | 402 |
| PAA | 45.6 ± 1.7 | 540 ± 60 | 1410 |
| NAA | 13.3 ± 1.0 | 1000 ± 210 | 222 |
