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Bicarbonate activation of the E. coli DHNA-CoA synthase (EcMenB). A, EcMenB activity titrated with bicarbonate; the solid line is the curve-fitting result using a saturation model (KD = 0.72 mm) under the assumption that there is a low background level of bicarbonate in the buffer. B, effects of various small anions on the activity of EcMenB. The concentration of the anions is 10 mm in B. Error bars indicate S.E.
