Skip to main content
. 2010 Jul 19;285(39):30159–30169. doi: 10.1074/jbc.M110.147702

FIGURE 6.

FIGURE 6.

Distribution and distinctive sequence conservation of type I and type II DHNA-CoA synthases. A, type I and type II DHNA-CoA synthases form distinct clusters in phylogenic analysis. The Bayesian phylogenetic tree of the proteins in the MenB family was generated from 163 sequences, whose abbreviations are listed in the supplemental material with their gene identification numbers. Proteins are colored according to phylum, and arcs indicate the main subfamilies. The type II DHNA-CoA synthases are shaded in pale yellow. B, type-specific conservation of eight amino acid residues in close proximity of the essential Asp-185 in MtbMenB. The sequences are selected to represent the gene diversity of the 163 identified MenB proteins in the phylogenetic analysis. The distinctively conserved residues are shaded in gray for type I enzymes and in red for type II enzymes. The blue arrows point to Gly-156 in EcMenB and Asp-185 in MtbMenB. A few EcMenB residues mentioned “under ”Results“ are numbered in the alignment. V. fischeri, Vibrio fischeri; B. licheniformis, Bacillus licheniformis; C. aurantiacus, Chloroflexus aurantiacus; T. elongatus, Thermosynechococcus elongatus; C. tepidum, Chlorobium tepidum; S. oneidensis, Shewanella oneidensis; B. bacteriovorus, Bdellovibrio bacteriovorus; H. marismortui, Haloarcula marismortui; N. pharaonis, Natronomonas pharaonis; N. farcinica, Nocardia farcinica; L. xyli, Leifsonia xyli.