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. 2010 Jul 22;285(39):29932–29940. doi: 10.1074/jbc.M110.131342

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — Transactivation activity of the wild type and mutant MRs. HEK-293T cells transiently expressing the MR, MR_N770A, MR_A773G, MR_Q776A, MR_S810A, MR_S810M, MR_R817A, MR_M852A, MR_C942A, MR_T945A, or MR_A773G/S810M were incubated for 16 h with aldosterone (Aldo), 18-vinyl-4-pregnen-3,18,20-trione (18OVP), or spironolactone (Spiro). The cell extracts were assayed for luciferase and β-galactosidase activities as reported previously (30). The GraphPad Prism software was used for curve fitting and calculation of the EC₅₀ values.