FIGURE 1.

Sequence alignment of mouse RIMKLA and RIMKLB with E. coli RIMK and E. coli glutathione synthase. The following sequences are shown: mouse RIMKLA (MmusA, NP_808240), mouse RIMKLB (MmusB, NP_081940), E. coli RIMK (EcRIM, NP_415373), E. coli glutathione synthetase (EcGlS, NP_417422, Protein Data Bank code 1GSH). Strictly conserved residues are indicated in boldface type. Asterisks indicate residues of the catalytic site involved in the binding of glutathione synthase substrates (see “Discussion”). The peptides used to prepare anti-RIMKLA and anti-RIMKLB antibodies are underlined.