FIGURE 1.

PPARγ agonists inhibit RANTES expression induced by TNF-α in rat glomerular mesangial cells. Mesangial cells were incubated with TNF-α (5 ng/ml) in the absence or presence of either endogenous (A and B) or synthetic PPARγ agonists (C and D) as indicated. Cells were treated either with 15d-PGJ2 (5 μm) for different periods of time (A) or various concentrations (B) for 48 h or with different doses of ciglitazone (C) and troglitazone (D) for 48 h. Cell lysates were immunoblotted with antibodies against RANTES or α-tubulin, respectively.