FIGURE 5.

A PrP-GPI-PSS synthetic peptide inhibits A7 cell spreading and migration. A, microscopic images show that incubation with a KKRPK-PrP-GPI-PSS synthetic peptide reduces A7 cell spreading. Arrows identify adherent cells. OM, 10×10. B, quantification of the cell spreading results. Results presented were the average ± S.E. of two experiments. OM, 10×10. C, quantification of a wound-healing assay at 15 h after the initiation of wound which shows that significant inhibition of cell migration could be achieved with as little as 0.5 μm concentrations of the peptide. Five images of every concentration were used to calculate the p values by analysis of variance one-way test. D, representative photographs of microscopic images taken at 15 h after initiation of wound, which show that incubation with a KKRPK-PrP-GPI-PSS synthetic peptide reduces A7 cell migration.