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. 2010 Jul 21;285(39):30026–30033. doi: 10.1074/jbc.M110.143545

FIGURE 1.

FIGURE 1.

Identification of prApe1- and Ams1-binding domains of Atg19 and Atg34. A, in vitro pulldown assay between three GST-fused domains of Atg19, the GST-fused C-terminal domain of Atg34, and Ams1. The input and eluted proteins were subjected to SDS-PAGE and detected by Coomassie Brilliant Blue staining. B, in vitro pulldown assay between GST-fused prApe1 propeptide (residues 1–20; Ape1n20) and the three domains of Atg19. The input and eluted proteins were subjected to SDS-PAGE and detected by Coomassie Brilliant Blue staining.