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Kinetic properties of Cyb5A/Cyb5R3 and Ncb5or-b5/Ncb5or-b5R reduction
The raw data of V0 versus [S] were fit to the Michaelis-Menten equation to generate Km and kcat (Vmax/[E]). The substrate is either Cyb5A or Ncb5or-b5, and the enzyme is either Cyb5R3 or Ncb5or-b5R.
| Substrate/Enzyme | [E] | Km | kcat | kcat/Km |
|---|---|---|---|---|
| nm | μm | s−1 | μm−1s−1 | |
| Cyb5A/Cyb5R3 | 0.56 | 7.6 ± 0.3 | 230 ± 3 | 30.1 |
| Ncb5or-b5/ Ncb5or-b5R | 140 | 498 ± 129 | 8.8 ± 1.9 | 0.018 |
