. 2010 Jul 14;285(39):30181–30191. doi: 10.1074/jbc.M110.120329

TABLE 3.

Initial rate (μm/min/μm) of interdomain electron transfer

Ncb5or-b₅ (wild type and W114A and R113A mutants) or Cyb5A (1.4 μm each) was reduced by Ncb5or-b₅R, Ncb5or-CS/b₅R, or Cyb5R3, in the presence of excess NADH (50 μm) in 5 mm sodium phosphate (pH 7.0, no air). All of the values in the table were normalized by reductase concentrations.

	Cyb5A	Ncb5or-b₅	Ncb5or-b₅W114A	Ncb5or-b₅R113A
Cyb5R3	1730 ± 63^a	24.60 ± 1.73^b	11.06 ± 1.23^b	24.52 ± 2.27^b
Ncb5or-b₅R	22.97 ± 1.67^b	5.69 ± 0.29^c	2.80 ± 0.22^c	4.63 ± 0.10^c
Ncb5or-CS/b₅R	0.24 ± 0.01^d	0.69 ± 0.06^d	0.17 ± 0.02^d	0.42 ± 0.04^d

^a 0.56 nm reductase used.

^b 28 nm reductase used.

^c 140 nm reductase used.

^d 500 nm reductase used.