Skip to main content
. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Nature. 2010 Jul 11;466(7306):612–616. doi: 10.1038/nature09172

Figure 1. The Z Amplicon.

Figure 1

a. Fluorescence in situ hybridization of Z-amplicon BAC CH261-77N6 (red) to distal long arm of Z chromosome (blue).

b. Z amplicon (red) comprises most distal 11 Mb of Z chromosome.

c. Triangular dot plots each comparing the sequence of a Z-chromosome BAC to itself. Within the plot, each dot represents a perfect match of 50 base pairs. Direct repeats appear as horizontal lines; scale bar represents 50kb. On left, BAC CH261-73L15 contains six tandem repeats covering 120 kb immediately proximal to Z amplicon. On right, BAC CH261-137P21, a representative Z amplicon clone. Each 25–30kb repeat unit is ~95% similar to any other, though some units have been disrupted by insertions and deletions.

d. Genes in repeat units of Z amplicon; scale bar represents 5kb. Each 20kb repeat unit of small array in CH261-73L15 contains one copy of ADCY10z. Each 25–30kb repeat unit of Z amplicon contains one copy each of C2ORF3z, MRPL19z, and RICSz.

e. RT-PCR analysis of Z-amplicon gene expression in adult tissues. HPRT1 is widely expressed in adult tissues and serves as positive control for reverse transcriptase reaction. All Z-amplicon genes are expressed in testis, but not other tissues.