. Author manuscript; available in PMC: 2010 Oct 1.

Published in final edited form as: Nat Protoc. 2010 Mar 25;5(4):702–713. doi: 10.1038/nprot.2010.11

Table 2.

List of antibodies for colony and sphere characterization

Antibody	Clone/Company	Dilution	Cell type stained
CK5	PRB-160P/Covance	1:1000	Single positive: basal and stem cells Double positive with CK8: trans-amplifying cells
CK8	MMS-162P/Covance	1:1000	Single positive: luminal cells Double positive with CK5: trans-amplifying cells
p63	sc-8431/Santa Cruz	1:200	Basal cells
AR	sc-816/Santa Cruz	1:200	Luminal cells
SMA	ab5694-100/AbCam	1:400	Stroma/smooth muscle cells
Vimentin	ab39376-100/AbCam	1:400	Stroma
Synaptophysin	MAB5258/Chemicon	1:200	Neuroendocrine cells