Figure 5.

Cocaine self-administration results in increased association of pCREB with BDNF exon IV in the mPFC. A, Western blots showing total pCREB and GAPDH levels in the mPFC. B, Analysis of the Western blot quantification showed no significant change in pCREB levels in mPFC in cocaine self-administration relative to saline controls (saline, n = 3; cocaine, n = 3). IDV, Integrated density value. C, However, ChIP analyses showed that there was a significant increase in the association of pCREB with BDNF promoter IV in the mPFC in the cocaine relative to the saline group (saline, n = 9; cocaine, n = 5) (Tukey's HSD test, *p < 0.05).