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. Author manuscript; available in PMC: 2010 Sep 21.
Published in final edited form as: J Exp Stroke Transl Med. 2010 Mar;3(1):28–33. doi: 10.6030/1939-067x-3.1.28

Figure 1. Ligation of the distal portion of MCA.

Figure 1

(A) Schematic depiction showing orientation of the animal in our model for induction of stroke. The red rectangle represents the portion shown in panel B. (B–E) After dissecting the left zygoma, a hole was made and the dura was carefully removed (B; lower magnification, C; higher magnification). The main trunk of the MCA was electrocoagulated (D) and disconnected using the tip of 30G needle (E). This mouse had a minor cortical branch (C), but electrocoagulation and disconnection of main trunk resulted in disappearance of blood flow in the minor cortical branch. Panel (F) shows a representative picture of the vascular structure without a minor cortical branch around olfactory tract. Scale bar: 1 mm (B) and 0.5 mm (C).