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Two-dimensional adipogenic-differentiated hASCs. hASCs were cultured for 12 days in the medium containing 10 μM insulin (A–D) or 1 μM insulin (E–H) (n = 3), and secreted glycerol (mg/mL) was measured at each time point (I). Scale bar = 50 μm. Original magnification, 10 × . Significant difference between insulin levels are labeled as +p < 0.05; significant differences from day 0 levels are labeled as *p < 0.05.
