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Triglyceride accumulation and secreted glycerol measurements of 3D constructs cultured in medium containing 10 μM or 1 μM insulin. (A, D) adipocyte/endothelial co-cultures, (B, E) adipocyte monocultures, and (C, F) endothelial monocultures were evaluated (n = 3). Significant differences from day 0 levels labeled as *p < 0.05.
