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Real time reverse transcription (RT)-PCR measurements for glut4 and CD31 transcript expression of 3D constructs cultured in medium containing 10 μM or 1 μM insulin. (A, D) adipocyte/endothelial co-cultures, (B, E) adipocyte monocultures, and (C, F) endothelial monocultures were evaluated (n = 3). Significant differences from day 0 levels are labeled as *p < 0.05.
