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Response to ACh in zero calcium in bioengineered tissue and rabbit longitudinal tissue. Native longitudinal tissue generated only 16.57 ± 2.095 μN (n = 6) in response to 1 μM ACh in the absence of extracellular calcium. This force was also sustained only for 2.5 min, versus the 12 min of force sustenance in regular calcium. Bioengineered tissue also showed force attenuated in magnitude by 50% and a reduction in time by 60%.
