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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Biochim Biophys Acta. 2010 Jun 19;1800(9):1018–1026. doi: 10.1016/j.bbagen.2010.06.007

FIGURE 4. Inhibition of NGF binding to TrkA.

FIGURE 4

NIH-TrkA cells were studied. (A) Scatchard plot analysis of high affinity 125I[NGF] binding data. Binding assays were carried out using a range of 125I[NGF] and a 100-fold excess of unlabeled NGF or a 500-fold excess of mimetic 1-ss as inhibitors. (B) Displacement of a constant concentration of 125I[NGF] (∼15,000 cpms) by increasing doses of unlabeled NGF or by mimetic 1-ss. In all assays NIH-3T3 wild type cells were used to assess nonspecific background (<15% of total binding).