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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Biochim Biophys Acta. 2010 Jun 19;1800(9):1018–1026. doi: 10.1016/j.bbagen.2010.06.007

FIGURE 5. NGF and 1-ss induce/stabilize cross-linking of TrkA dimers.

FIGURE 5

NIH-TrkA cells were studied. After exposure to the indicated ligand (30 min at 4°C), or to no ligand, cells were chemically cross-linked with DSS. After washing, cells were detergent solubilized and samples (20 μg protein/lane) were studied by western blotting with a highly specific anti-TrkA mAb 5C3. The amount of putative TrkA dimer (280 kDa) relative to optimal NGF-induced dimer (100%) is indicated. Note that untreated NIH-TrkA cells have ∼32% of the level of cross-linked TrkA dimers fond in NGF-treated cells.