Skip to main content
. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Biochim Biophys Acta. 2010 Jun 19;1800(9):1018–1026. doi: 10.1016/j.bbagen.2010.06.007

FIGURE 6. Mimetic 1-ss-TEG and 1-ss-fluorescein antagonize NGF and TrkA in cell survival assays.

FIGURE 6

NIH-3T3 cells expressing TrkA or TrkC, or PC12 cells were cultured in SFM alone or supplemented with growth factor (NGF for TrkA-expressing cells, NT-3 for TrkC-expressing cells). Survival was measured by MTT assays, and was calculated relative to optimal neurotrophin (100% protection). Suboptimal concentrations of growth factor (0.2 nM or 0.1 nM) were used to achieve limited survival. Results shown are average ± SEM, from at least three independent experiments (n = 4 per experiment). (A) Mimetic 1-ss-TEG (20 μM) antagonizes 0.2 nM NGF; but not (B) 0.1 nM NT-3. (C) Dose-dependent antagonism of TrkA-NGF by increasing doses of 1-ss-TEG in NIH-TkA TkA cells over-expressing TrkA. (D) Increasing doses of NGF oppose the antagonism of a constant dose of 1-ss-TEG (20 μM) in NIH-TkA cells over-expressing TrkA. (E) Antagonism of optimal (10 nM) NGF–induced survival in PC12 cells, expressing low levels of TrkA, using 20 μM 1-ss-TEG or 1-ss-fluorescein.