. Author manuscript; available in PMC: 2011 Sep 1.

Published in final edited form as: Biochim Biophys Acta. 2010 Jun 19;1800(9):1018–1026. doi: 10.1016/j.bbagen.2010.06.007

TABLE 1.

Representative 3-day MTT assay of cell survival. The indicated cells were cultured in SFM in 96-well plates (n=6 wells/condition). The indicated wells were supplemented with serum (normal culture: growth + survival), or neurotrophin (NTF: NGF for TrkA-expressing cells, NT-3 for TrkC-expressing cells); with or without 1-ss-TEG (20 μM). The relative resistance to death in SFM is reduced by 1-ss-TEG in NIH-TrkA but not in NIH-TrkC. Ligand-independent survival is not seen in PC12 cells, as these cells do not over-express TrkA. Furthermore, the survival promoted by NGF is reduced by 1-ss-TEG in NIH-TrkA and PC12 cells, but the survival promoted by NT-3 is not reduced in NIH-TrkC.

TREATMENT	OD₄₉₀ (×1000) values
	TrkA-NIH	TrkC-NIH	PC12
5% serum	609 ± 28	706 ± 61	403 ± 13
5% serum + 1-ss-TEG	611 ± 22	700 ± 34	398 ± 10
NTF optimal (10 nM)	464 ± 12	535 ± 16	309 ± 8
NTF suboptimal (0.2 or 0.1 nM)	243 ± 17	269 ± 23	145 ± 25
SMF	127 ± 7	153 ± 4	29 ± 4
1-ss-TEG	59 ± 5	156 ± 5	32 ± 5
NTF suboptimal + 1-ss-TEG	155 ± 11	274 ± 19	42 ± 6