Figure 4.

Analysis of moving neurofilaments in wild-type and dilute lethal axons. Histograms of frequency of movement (A, B), average velocities (including and excluding pauses) (C–F), and peak velocities (G, H) of neurofilaments in wild-type (n = 58) and DLS/LeJ dilute lethal (n = 53) axons. In total, we imaged 27 gaps in axons from three different batches of wild-type cultures and 21 gaps in axons from three different batches of dilute lethal cultures. The average length of the time-lapse movies was 13.3 min. Anterograde and retrograde movements are represented on the y-axis as positive and negative values, respectively. The average velocity including pauses represents the overall average for all the time intervals in which the neurofilament was tracked. Average velocity excluding pauses represents the average for only those time intervals in which the neurofilament moved. Statistical comparison of these data revealed no significant difference between wild type and dilute lethal for either direction of movement (supplemental Table 1, available at www.jneurosci.org as supplemental material).