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. Author manuscript; available in PMC: 2011 Sep 24.
Published in final edited form as: Chem Biol. 2010 Sep 24;17(9):981–988. doi: 10.1016/j.chembiol.2010.07.009

Figure 2. FKBP- and ecDHFR-derived DDs are tunably and predictably regulated using their respective ligands.

Figure 2

(A) NIH3T3 cells doubly transduced with ecDHFR-derived R12Y/Y100I-YFP and the FKBP-derived L106P-mCherry were dosed with varying concentrations of TMP (black) and Shield-1 (red), and fluorescence was monitored by flow cytometry at the time points indicated. Predicted MFI values (thick lines) were generated using a previous dose-response study of the same cell line. Data are presented as the average MFI ± s.d. and normalized against the average MFI of cells dosed with 10 μM TMP and 3 μM Shield-1, which were set at 100%. (B) NIH3T3 cells described in panel A were plated onto 2-well chamber slides and treated for 24 h with concentrations of TMP and Shield-1 corresponding to the conditions used in panel B. Live cells were visualized by epifluorescence microscopy.