FIG. 2.
Coherent anti-Stokes Raman spectroscopy (CARS) and immunohistochemistry images of normal and damaged myelin in ventral white matter strips. (A) CARS image of normal myelin. A node of Ranvier is highlighted in the dashed box. (B) Immunofluorescent image of fast potassium voltage channels Kv1.2 clustered in the juxtaparanodal area. (C) Merged image of CARS (A) and immunofluorescence (B) depicting Kv1.2 channels that are normally shielded by myelin. (D) CARS image of damaged myelin and a node of Ranvier. The dashed white box delineates the node of Ranvier. (E) Immunofluorescence of Kv1.2 channels. (F) Merged image of CARS (D) and immunostaining (E) showing partial Kv1.2 exposure following 80% sustained compression. (G) The node ratio was used to quantify elongation of the nodal region. The node ratio was defined as the nodal distance divided by the nodal diameter. Larger node ratios indicate more myelin disruption (scale bar = 5 μm). (H) Scatterplot of the node ratio for compressed (30 min sustained) and uninjured cord as a function of axon diameter. Node morphology analysis showed an increase in the node ratio following compression. (Color image is available online at www.liebertonline.com/neu.)