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. Author manuscript; available in PMC: 2011 Jul 15.
Published in final edited form as: Cancer Res. 2010 Jun 22;70(14):5901–5911. doi: 10.1158/0008-5472.CAN-10-0192

Figure. 3. Cell lines harboring an A146T KRAS mutation demonstrate elevated RAS-GTP expression and KRAS-dependence.

Figure. 3

A. HEK-293FT cells were transfected with KRAS mutants and GTP-bound RAS was measured by immuno-precipitating active RAS with recombinant Ras binding domain (RBD) of RAF. B. Colorectal cancer cell lines expressing A146T KRAS demonstrate elevated RAS-GTP expression as compared to cell lines with V600E BRAF mutation or those wild-type for both KRAS and BRAF. C. Knockdown of KRAS expression by siRNA in LS1034 cells (homozygous A146T KRAS mutant) inhibited colony formation.