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Separation of DdeI digestion products (2.5% agarose gel, reverse contrast). Lanes 1 and 11: 100-bp ladder; lanes 2 and 3: normal dogs; lanes 4 and 5: heterozygous carriers; lanes 6–9: PMDS-affected dogs; lane 10: undigested 662-bp PCR product. The 3 diagnostic bands are indicated by asterisks: 333 bp (*), 255 bp (**) and 212 bp (***). Dog identification numbers are above the bands and genotypes at the C241T site are below the bands.
