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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Cell Microbiol. 2010 Jun 16;12(11):1576–1588. doi: 10.1111/j.1462-5822.2010.01490.x

Figure 4.

Figure 4

(A) HBMEC monolayers were treated with signaling pathway inhibitors TCPK, SB202190 and U0126 for 30 min prior to infection with SPN. Protein expression of IL-8 in hBMEC supernatants, 6 h post infection was determined using ELISA. Quantification of bacterial invasion following hBMEC treatment with U0126 (30 min) compared to DMSO control (B), TNFα and NanA-GST (C) or NanA-GST and NanAΔEnz-GST (D). Experiments were performed three times in triplicate. Bars represent mean and standard deviation of one representative experiment. ** p<0.005, *** p<0.001.